- Laboratories Dental, Ankeny
- Laboratories Medical, Ankeny
- Land Surveyor, Ankeny - Land Surveying Services
- Landscape Contractors & Designers, Ankeny
- Landscaping, Ankeny
- Landscaping Materials & Supplies, Ankeny
- Late Night Restaurants, Ankeny
- Laundry Self Service, Ankeny
- Lawn & Garden Equipment & Supplies, Ankeny
- Lawn & Garden Services, Ankeny
- Lawn Maintenance Services, Ankeny
- Lawn Mowers & Power Equipment, Ankeny
- Lawyers & Attorneys, Ankeny
- Leather Goods Cleaners & Dyers, Ankeny
- Legal & Tax Services, Ankeny
- Legal Information Service, Ankeny
- Legal Professionals, Ankeny
- License Bureaus Motor Vehicles, Ankeny
- Life Insurance, Ankeny
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- Lighting Fixtures & Equipment, Ankeny
- Lighting Fixtures Repair, Maintenance & Installation, Ankeny
- Lighting Maintenance, Ankeny
- Liquor Stores, Ankeny
- Livestock, Ankeny
- Livestock Equipment & Supplies, Ankeny
- Livestock Services, Ankeny
- Living Room Furniture, Ankeny
- Loans Check Cashing, Ankeny
- Loans Personal, Ankeny
- Loans Title Services, Ankeny
- Lodging, Ankeny
- Lodging Services, Ankeny
- Logistics Freight, Ankeny
- Long Haul Trucking Companies, Ankeny
- Lumber & Lumber Products, Ankeny
- Lumber & Lumber Products, Ankeny
- Lutheran Church, Ankeny
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